| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Voloshin, Emily S (10)
|
NJLHY |
42.21 |
39.09 |
-3.12 |
| 2 |
Galloway, Regan D (9)
|
NJRANY |
48.06 |
43.01 |
-5.05 |
| 3 |
Oei, Samantha G (10)
|
NJSHY |
49.12 |
43.38 |
-5.74 |
| 4 |
Gao, Jenny (10)
|
NJGMY |
41.67 |
43.45 |
1.78 |
| 5 |
Patchett, Clare M (10)
|
NJSHY |
49.21 |
44.39 |
-4.82 |
| 6 |
McLaughlin, Madelyn P (9)
|
NJRANY |
46.47 |
44.75 |
-1.72 |
| 7 |
DE Peralta, Jilliane A (9)
|
NJGMY |
44.24 |
45.09 |
0.85 |
| 8 |
Patel, Ayana (9)
|
NJGMY |
49.78 |
45.22 |
-4.56 |
| 9 |
Georgieva, Sophia G (10)
|
NJGMY |
52.62 |
46.38 |
-6.24 |
| 10 |
Falcone, Kaycie A (9)
|
NJRANY |
47.90 |
46.78 |
-1.12 |
| 11 |
Hornos, Milena C (10)
|
NJSHY |
50.81 |
48.63 |
-2.18 |
| 12 |
Strangeway, Quinn M (10)
|
NJRANY |
49.81 |
49.00 |
-0.81 |
| 13 |
Yiu, Ava C (10)
|
NJSHY |
55.00 |
50.26 |
-4.74 |
| 14 |
Sanders, Sophie G (9)
|
NJRANY |
57.57 |
51.89 |
-5.68 |
| 15 |
DiBello, Nicole (9)
|
NJRANY |
1:00.41 |
59.75 |
-0.66 |
| 16 |
Bogert, Emily L (9)
|
NJGMY |
1:01.62 |
1:01.83 |
0.21 |